Open AccessIn Vivo Notch Signaling Blockade Induces Abnormal Spermatogenesis in the Mouse
Author(s) -
Daniel Murta,
Marta Batista,
Alexandre Trindade,
Elisabete Silva,
Domingos Henrique,
António Duarte,
Luís Lopes-da-Costa
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0113365
Subject(s) - notch signaling pathway , spermatogenesis , epididymis , biology , germ cell , microbiology and biotechnology , sertoli cell , blockade , signal transduction , endocrinology , receptor , genetics , gene , sperm
In a previous study we identified active Notch signaling in key cellular events occurring at adult spermatogenesis. In this study, we evaluated the function of Notch signaling in spermatogenesis through the effects of in vivo Notch blockade. Adult CD1 male mice were either submitted to a long term DAPT (?-secretase inhibitor) or vehicle treatment. Treatment duration was designed to attain one half the time (25 days) or the time (43 days) required to accomplish a complete cycle of spermatogenesis. Blockade of Notch signaling was depicted from decreased transcription of Notch effector genes. Notch signaling blockade disrupted the expression patterns of Notch components in the testis, induced male germ cell fate aberrations, and significantly increased germ cell apoptosis, mainly in the last stages of the spermatogenic cycle, and epididymis spermatozoa morphological defects. These effects were more pronounced following the 43 day than the 25 day DAPT treatment schedule. These results indicate a relevant regulatory role of Notch signaling in mammalian spermatogenesis.