Open AccessCSF Proteomics of Secondary Phase Spinal Cord Injury in Human Subjects: Perturbed Molecular Pathways Post Injury
Author(s) -
Mohor Biplab Sengupta,
Mahashweta Basu,
Sourav Iswarari,
Kiran Kumar Mukhopadhyay,
Krishna Pada Sardar,
Biplab Acharyya,
P. K. Mohanty,
Debashis Mukhopadhyay
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0110885
Subject(s) - spinal cord injury , proteomics , biology , traumatic brain injury , medicine , phosphorylation , spinal cord , microbiology and biotechnology , bioinformatics , gene , neuroscience , biochemistry , psychiatry
Recovery of sensory and motor functions following traumatic spinal cord injury (SCI) is dependent on injury severity. Here we identified 49 proteins from cerebrospinal fluid (CSF) of SCI patients, eight of which were differentially abundant among two severity groups of SCI. It was observed that the abundance profiles of these proteins change over a time period of days to months post SCI. Statistical analysis revealed that these proteins take part in several molecular pathways including DNA repair, protein phosphorylation, tRNA transcription, iron transport, mRNA metabolism, immune response and lipid and ATP catabolism. These pathways reflect a set of mechanisms that the system may adopt to cope up with the assault depending on the injury severity, thus leading to observed physiological responses. Apart from putting forward a picture of the molecular scenario at the injury site in a human study, this finding further delineates consequent pathways and molecules that may be altered by external intervention to restrict neural degeneration.