z-logo
open-access-imgOpen Access
Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice
Author(s) -
Sabry M. Attia,
Sheikh F. Ahmad,
Radwa Mohamed Okash,
Saleh A. Bakheet
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0109942
Subject(s) - clastogen , aneuploidy , biology , micronucleus test , somatic cell , microbiology and biotechnology , epirubicin , sperm , meiosis , fluorescence in situ hybridization , genetics , andrology , chemistry , chromosome , medicine , cancer , toxicity , breast cancer , gene , organic chemistry
The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo . By using the BrdU-incorporation assay, it could be shown that the meiotic delay caused by epirubicin in germ cells was approximately 48 h. Disomic and diploid sperm were shown in epididymal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XY-sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise to the development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical personnel exposed to epirubicin.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here