Open AccessIdentification of Trueperella pyogenes Isolated from Bovine Mastitis by Fourier Transform Infrared Spectroscopy
Author(s) -
Samy Nagib,
Jörg Rau,
Osama Sammra,
Christoph Lämmler,
Karen Schlez,
M. Zschöck,
Ellen PrengerBerninghoff,
G. Klein,
Amir Abdulmawjood
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0104654
Subject(s) - biology , fourier transform infrared spectroscopy , mastitis , virulence , microbiology and biotechnology , genotype , gene , genetics , optics , physics
The present study was designed to investigate the potential of Fourier transform infrared (FT-IR) spectroscopy to identify Trueperella ( T. ) pyogenes isolated from bovine clinical mastitis. FT-IR spectroscopy was applied to 57 isolates obtained from 55 cows in a period from 2009 to 2012. Prior to FT-IR spectroscopy these isolates were identified by phenotypic and genotypic properties, also including the determination of seven potential virulence factor encoding genes. The FT-IR analysis revealed a reliable identification of all 57 isolates as T. pyogenes and a clear separation of this species from the other species of genus Trueperella and from species of genus Arcanobacterium and Actinomyces . The results showed that all 57 isolates were assigned to the correct species indicating that FT-IR spectroscopy could also be efficiently used for identification of this bacterial pathogen.