Open AccessThe Metabolism of Salidroside to Its Aglycone p-Tyrosol in Rats following the Administration of Salidroside
Author(s) -
Na Guo,
Meixuan Zhu,
Xuejiao Han,
Dan Sui,
Yang Wang,
Yueming Qian
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0103648
Subject(s) - salidroside , tyrosol , aglycone , chemistry , urine , pharmacology , oral administration , kidney , rhodiola rosea , biochemistry , medicine , glycoside , chromatography , endocrinology , phenols , stereochemistry
Salidroside is one of the major phenolic glycosides in Rhodiola , which has been reported to possess various biological activities. In the present study the in vivo deglycosylation metabolism of salidroside was investigated and its aglycone p -tyrosol but not the original salidroside was identified as the main form in rat tissues following the administration of salidroside. After the i.v. administration of salidroside at a dose of 50 mg/kg in rats, salidroside was quantified only in the liver, kidney and heart tissues. The highest level of p -tyrosol was detected in the heart, followed by the spleen, kidney, liver and lungs, in order. Salidroside was detected only in the liver, in contrast, p- tyrosol was detectable in most tissues except the brain, and the kidney tissues contained a significant amount of p- tyrosol compared to the other tissues after the i.g. administration of 100 mg/kg salidroside. The excretion behaviour revealed that the administrated salidroside mainly eliminated in the form of salidroside but not its aglycone metabolite p -tyrosol through urine. After i.v. and i.g. administration in rats, 64.00% and 23.80% of the total dose was excreted through urine in the form of salidroside, respectively. In addition, 0.19% and 2.25% of the dose was excreted in the form of p- tyrosol through urine after i.v. and i.g. administration, respectively. The faecal salidroside and p -tyrosol concentrations were 0.3% and 1.48% of the total dose after i.v. administration, respectively. After the i.g. administration of salidroside, trace salidroside and p -tyrosol were quantified in faeces within 72 h. In addition, the biliary excretion levels of salidroside after i.v. and i.g. administration were 2.86% and 0.02% of the dose, respectively. The obtained results show that salidroside was extensively metabolised to its aglycone p -tyrosol and distributed to various organs and the orginal salidroside was cleared rapidly through urine following the administration of salidroside.