Open AccessSynergistic Antifungal Effect of Glabridin and Fluconazole
Author(s) -
Wei Liu,
Li Ping Li,
Jun Dong Zhang,
Qun Li,
Hui Shen,
Si Min Chen,
Li He,
Lan Yan,
Guo Xu,
Mao Mao An,
Yuan Jiang
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0103442
Subject(s) - candida albicans , candida tropicalis , candida krusei , candida parapsilosis , fluconazole , minimum inhibitory concentration , cryptococcus neoformans , microbiology and biotechnology , chemistry , corpus albicans , antifungal , biology , pharmacology , antimicrobial
The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species ( Candida albicans , Candida tropicalis , Candida krusei , Candida parapsilosis and Candida Glabratas ) by different methods. The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) indicated that Gla possessed a broad-spectrum antifungal activity at relatively high concentrations. After combining with FLC, Gla exerted a potent synergistic effect against drug-resistant C. albicans and C. tropicalis at lower concentrations when interpreted by fractional inhibitory concentration index (FICI). Disk diffusion test and time-killing test confirming the synergistic fungicidal effect. Cell growth tests suggested that the synergistic effect of the two drugs depended more on the concentration of Gla. The cell envelop damage including a significant decrease of cell size and membrane permeability increasing were found after Gla treatment. Together, our results suggested that Gla possessed a synergistic effect with FLC and the cell envelope damage maybe contributed to the synergistic effect, which providing new information for developing novel antifungal agents.