Open AccessVanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
Author(s) -
Milan Žižić,
Miroslav Živić,
Vuk Maksimović,
Marina Stanić,
Strahinja Križak,
Tijana Cvetić Antić,
Joanna Zakrzewska
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0102849
Subject(s) - phycomyces blakesleeanus , vanadate , sugar phosphates , biochemistry , phycomyces , glycogen phosphorylase , phosphate , chemistry , fructose , dihydroxyacetone phosphate , metabolism , glycogen , biology , mutant , gene
The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V 5+ ) on phosphate metabolism of Phycomyces blakesleeanus . Addition of V 5+ caused increase of sugar phosphates signal intensities in 31 P NMR spectra in vivo . HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V 5+ treatment. Influence of V 5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP ( 31 P NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V 4+ ), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.