Open AccessDetecting DNA Depurination with Solid-State Nanopores
Author(s) -
Michael M. Marshall,
Jan Růžička,
Ethan Will Taylor,
Adam R. Hall
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0101632
Subject(s) - depurination , ap site , dna , nanopore , mutagenesis , chemistry , biophysics , dna repair , biochemistry , biology , microbiology and biotechnology , nanotechnology , mutation , materials science , gene
Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dynamics during electrokinetic translocation. This procedure may be valuable as a diagnostic for in situ quantification of DNA depurination.