Open AccessMucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination
Author(s) -
Krzysztof Hinc,
Małgorzata Stasiłojć,
Iwona Piątek,
Grażyna Peszyńska-Sularz,
Rachele Isticato,
Ezio Ricca,
Michał Obuchowski,
Adam Iwanicki
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0095187
Subject(s) - bacillus subtilis , spore , microbiology and biotechnology , adjuvant , helicobacter pylori , recombinant dna , heterologous , vaccination , biology , immunization , immune system , plasmid , helicobacter , gene , immunology , bacteria , genetics
The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.