2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination | Zendy

Qifeng Li | Zendy; Yang Wang | Zendy; Da Chen | Zendy; Sherry Wu | Zendy

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2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination

Author(s) -

Qifeng Li,

Yang Wang,

Da Chen,

Sherry Wu

Publication year - 2014

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0088464

Subject(s) - sted microscopy , sapphire , laser , optics , stimulated emission , materials science , microscopy , fluorescence microscope , ti:sapphire laser , super resolution microscopy , optoelectronics , fluorescence , physics

We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.

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