Open Access2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
Author(s) -
Qifeng Li,
Yang Wang,
Da Chen,
Sherry Wu
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0088464
Subject(s) - sted microscopy , sapphire , laser , optics , stimulated emission , materials science , microscopy , fluorescence microscope , ti:sapphire laser , super resolution microscopy , optoelectronics , fluorescence , physics
We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.