Open AccessSPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis
Author(s) -
Elvire Beleoken,
Hervé Leh,
Armelle Arnoux,
Béatrice Ducot,
Claude Noguès,
Eléonora De Martin,
Catherine Johanet,
Didier Samuel,
Mohammad Zahid Mustafa,
JeanCharles DuclosVallée,
Malcolm Buckle,
Éric Ballot
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0084600
Subject(s) - anti nuclear antibody , autoantibody , heterogeneous nuclear ribonucleoprotein , immunology , ribonucleoprotein , autoimmune hepatitis , antibody , rheumatoid arthritis , lupus erythematosus , autoimmunity , medicine , small nuclear ribonucleoprotein , biomarker , chemistry , hepatitis , rna , biochemistry , gene
Background Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH).Aim To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and sera from patients and healthy controls. Methods Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants k off for each interaction were calculated. Results Several significant interactions were observed: i) high stability (lower k off values) between P 55-70 and the AIH sera compared to controls (p= 0.003); ii) lower stability (higher k off values) between P 118-133 and P 262-277 and SLE sera, P 145-160 and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and k off values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P 55-70 and also between controls and the peptides studied. Conclusions These results indicate that P 55-70 of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability.