Open AccessSNAP-Tag Technology Optimized for Use in Entamoeba histolytica
Author(s) -
Adam Sateriale,
Nathan H. Roy,
Christopher D. Huston
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0083997
Subject(s) - entamoeba histolytica , entamoeba , endoplasmic reticulum , biology , computational biology , microbiology and biotechnology
Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica . The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E . histolytica.