Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora | Zendy

François Cesbron | Zendy; Michael Brunner | Zendy; Axel Diernfellner | Zendy

Open Access

Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora

Author(s) -

François Cesbron,

Michael Brunner,

Axel Diernfellner

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0083660

Subject(s) - luciferase , neurospora , circadian clock , transcription factor , transcription (linguistics) , biology , in vivo , circadian rhythm , firefly protocol , bioluminescence , microbiology and biotechnology , genetics , biochemistry , gene , evolutionary biology , neuroscience , transfection , linguistics , philosophy , mutant , neurospora crassa

We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora , which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format.

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