Open AccessInduced Autologous Stem Cell Transplantation for Treatment of Rabbit Renal Interstitial Fibrosis
Author(s) -
GuoRui Ruan,
Fan Xu,
Zi-An Li,
Guangxu Zhu,
RongQing Pang,
Jinxiang Wang,
Xue-Min Cai,
Jie He,
Yang Xiang,
Guang-Hong Ruan,
Xiang Xu,
Xinghua Pan
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0083507
Subject(s) - transplantation , fibrosis , blood urea nitrogen , stem cell , renal function , creatinine , medicine , renal stem cell , kidney , pathology , urology , biology , microbiology and biotechnology , progenitor cell
Renal interstitial fibrosis (RIF) is a significant cause of end-stage renal failure. The goal of this study was to characterize the distribution of transplanted induced autologous stem cells in a rabbit model of renal interstitial fibrosis and evaluate its therapeutic efficacy for treatment of renal interstitial fibrosis. Methods A rabbit model of renal interstitial fibrosis was established. Autologous fibroblasts were cultured, induced and labeled with green fluorescent protein (GFP). These labeled stem cells were transplanted into the renal artery of model animals at 8 weeks. Results Eight weeks following transplantation of induced autologous stem cells, significant reductions ( P < 0.05) were observed in serum creatinine (SCr) (14.8 ± 1.9 mmol/L to 10.1 ± 2.1 mmol/L) and blood urea nitrogen (BUN) (119 ± 22 µmol/L to 97 ± 13 µmol/L), indicating improvement in renal function. Conclusions We successfully established a rabbit model of renal interstitial fibrosis and demonstrated that transplantation of induced autologous stem cells can repair kidney damage within 8 weeks. The repair occurred by both inhibition of further development of renal interstitial fibrosis and partial reversal of pre-existing renal interstitial fibrosis. These beneficial effects lead to the development of normal tissue structure and improved renal function.