Open AccessComparative Proteomic Analysis Reveals the Cross-Talk between the Responses Induced by H2O2 and by Long-Term Rice Black-Streaked Dwarf Virus Infection in Rice
Author(s) -
Qiufang Xu,
Haiping Ni,
Qingqing Chen,
Feng Sun,
Tong Zhou,
Ying Lan,
Yijun Zhou
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0081640
Subject(s) - biology , proteome , virus , proteomics , black spot , spots , photosynthesis , oxidative stress , rice plant , plant virus , microbiology and biotechnology , virology , botany , genetics , biochemistry , gene , horticulture
Hydrogen peroxide (H 2 O 2 ) could be produced during the plant-virus compatible interaction. However, the cell responses regulated by the enhanced H 2 O 2 in virus infected plant are largely unknown. To make clear the influence of Rice black-streaked dwarf virus (RBSDV) infection on H 2 O 2 accumulation, we measured the content of H 2 O 2 and found the H 2 O 2 level was increased in rice seedlings inoculated with RBSDV. To reveal the responses initiated by the enhanced H 2 O 2 during plant-virus interaction, the present study investigated the global proteome changes of rice under long-term RBSDV infection. Approximately 1800 protein spots were detected on two-dimensional electrophoresis (2-DE) gels. Among them, 72 spots were found differently expressed, of which 69 spots were successfully identified by MALDI-TOF/TOF-MS. Furthermore, the differentially expressed proteins induced by RBSDV infection were compared to that induced by H 2 O 2 . 19 proteins corresponding to 37 spots, which were differentially expressed under RBSDV infection, were observed differentially expressed under H 2 O 2 stress as well. These overlapping responsive proteins are mainly related to photosynthesis, redox homeostasis, metabolism, energy pathway, and cell wall modification. The increased H 2 O 2 in RBSDV infected plant may produce an oxidative stress, impair photosynthesis, disturb the metabolism, and eventually result in abnormal growth. The data provide a new understanding of the pivotal role of H 2 O 2 in rice-RBSDV compatible interaction.