Open AccessC. elegans Aging Is Modulated by Hydrogen Sulfide and the sulfhydrylase/cysteine Synthase cysl-2
Author(s) -
Bedoor Qabazard,
Samanza Ahmed,
Ling Li,
Volker M. Arlt,
Philip K. Moore,
Stephen R. Stürzenbaum
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0080135
Subject(s) - oxidative stress , atp synthase , endogeny , endoplasmic reticulum , microbiology and biotechnology , cystathionine beta synthase , longevity , cystathionine gamma lyase , chemistry , unfolded protein response , downregulation and upregulation , cysteine , biochemistry , biology , enzyme , genetics , gene
Exogenous hydrogen sulfide (H 2 S) administration and endogenous H 2 S metabolism were explored in the nematode C. elegans . Chronic treatment with a slow-releasing H 2 S donor, GYY4137, extended median survival by 17-23% and increased tolerance towards oxidative and endoplasmic reticulum (ER) stress. Also, cysl-2 , a sulfhydrylase/cysteine synthase in C. elegans , was transcriptionally upregulated by GYY4137 treatment and the deletion of cysl-2 resulted in a significant reduction in lifespan which was partially recovered by the supplementation of GYY4137. Likewise, a mammalian cell culture system, GYY4137 was able to protect bovine aortic endothelial cells (BAECs) from oxidative stress and (H 2 O 2 )-induced cell death. Taken together, this provides further support that H 2 S exerts a protective function which is consistent with the longevity dividend theory. Overall, this study underlines the therapeutic potential of a slow-releasing H 2 S donor as regulators of the aging and cellular stress pathways.