Open AccessNitrotyrosine Level Was Associated with Mortality in Patients with Acute Kidney Injury
Author(s) -
Ji Qian,
Huaizhou You,
Qiuyu Zhu,
Shuai Ma,
Ying Zhou,
Zheng Ying,
Junfeng Liu,
Dingwei Kuang,
Yong Gu,
ChuanMing Hao,
Feng Ding
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0079962
Subject(s) - medicine , rifle , acute kidney injury , sepsis , nitrotyrosine , proportional hazards model , gastroenterology , prospective cohort study , kidney disease , nitric oxide , nitric oxide synthase , archaeology , history
Background To examine the characteristics of oxidative stress in patients with acute kidney injury (AKI) and investigate the association between plasma nitrotyrosine levels and 90-day mortality in patients with AKI. Methodology/Principal Findings 158 patients with hospital-acquired AKI were recruited to this prospective cohort study according to RIFLE (Risk, Injury, Failure, Lost or End Stage Kidney) criteria. Twelve critically ill patients without AKI and 15 age and gender-matched healthy subjects served as control. Plasma 3-nitrotyrosine was analyzed in relation to 90-day all cause mortality of patients with AKI. The patients with AKI were followed up for 90 days and grouped according to median plasma 3-nitrotyrosine concentrations. Highest 3-NT/Tyr was detected in patients with AKI compared with healthy subjects, and critically ill patients without AKI (ANOVA p <0.001). The 90-day survival curves of patients with high 3-NT/Tyr showed significant differences compared with the curves of individuals with low 3-NT/Tyr ( p = 0.001 by log rank test). Multivariate analysis (Cox regression) revealed that 3-NT/Tyr ( p = 0.025) was independently associated with mortality after adjustment for age, gender, sepsis and Acute Physiology and Chronic Health Evaluation (APACHE) II score. Conclusions/Significance There is excess plasma protein oxidation in patients with AKI, as evidenced by increased nitrotyrosine content. 3-NT/Tyr level was associated with mortality of AKI patients independent of the severity of illness.