Open AccessForming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression
Author(s) -
Gourapura J. Renukaradhya,
Masood Alam Khan,
Richard M. Gallo,
Daniel Shaji,
Jianyun Liu,
Randy R. Brutkiewicz
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0072867
Subject(s) - cd1d , cd1 , antigen presentation , mhc class i , antigen processing , biology , microbiology and biotechnology , mhc class ii , major histocompatibility complex , natural killer t cell , antigen , mhc restriction , antigen presenting cell , t cell , immunology , immune system , cd8
CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.