Open AccessEvidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
Author(s) -
Igor Dozmorov,
Nicolás Domínguez,
Andrea L. Sestak,
Julie M. Robertson,
John B. Harley,
Judith A. James,
Joel M. Guthridge
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0071397
Subject(s) - gene expression , gene expression profiling , regulation of gene expression , biology , gene , immune system , gene regulatory network , systemic lupus erythematosus , transcription factor , computational biology , lupus erythematosus , microbiology and biotechnology , genetics , immunology , medicine , antibody , pathology , disease
Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences between healthy controls and autoimmune patients. Here we apply an original systematic approach to the analysis of changes in regulatory gene interconnections between in Epstein-Barr virus transformed hyperresponsive B cells from SLE patients and normal control B cells. Both traditional analysis of differential gene expression and analysis of the dynamics of gene expression variations were performed in combination to establish model networks of functional gene expression. This Pathway Dysregulation Analysis identified known transcription factors and transcriptional regulators activated uniquely in stimulated B cells from SLE patients.