Open AccessGenerating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells
Author(s) -
Yanting Xue,
Xiujuan Cai,
Linli Wang,
Baojian Liao,
Hui Zhang,
Yongli Shan,
Qianyu Chen,
Tiancheng Zhou,
Xirui Li,
J. Hou,
Shubin Chen,
Rongping Luo,
Dajiang Qin,
Duanqing Pei,
Guangjin Pan
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0070573
Subject(s) - induced pluripotent stem cell , reprogramming , regenerative medicine , cell , population , biology , computational biology , cell culture , stem cell , drug discovery , microbiology and biotechnology , bioinformatics , embryonic stem cell , genetics , medicine , gene , environmental health
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC . We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.