Open AccessSilicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
Author(s) -
E A Bogdanov,
И. Н. Доминова,
Natalia Shusharina,
Stepan Botman,
Vitaliy Kasymov,
М. В. Патрушев
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0068280
Subject(s) - nucleic acid , dna , isolation (microbiology) , silicon dioxide , silicon , materials science , adsorption , cell , nanotechnology , chemistry , biophysics , biochemistry , biology , optoelectronics , bioinformatics , organic chemistry , composite material
A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.