Open AccessVaccination of Chickens with SPI1-lon and SPI1-lon-fliC Mutant of Salmonella enterica Serovar Enteritidis
Author(s) -
Marta Matulová,
Hana Havlíčková,
František Šišák,
Ivan Rychlı́k
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0066172
Subject(s) - serotype , salmonella enterica , flock , biology , salmonella enteritidis , vaccination , virology , microbiology and biotechnology , immunogenicity , flagellin , serology , attenuated vaccine , salmonella , immune system , virulence , antibody , genetics , gene , bacteria , paleontology
The prevalence of Salmonella enterica serovar Enteritidis is gradually decreasing in poultry flocks in the EU, which may result in the demand for a vaccine that allows for the differentiation of vaccinated flocks from those infected by wild-type S . Enteritidis. In this study, we therefore constructed a (Salmonella Pathogenicity Island 1) SPI1- lon mutant with or without fliC encoding for S . Enteritidis flagellin. The combination of SPI1- lon mutations resulted in attenuated but immunogenic mutant suitable for oral vaccination of poultry. In addition, the vaccination of chickens with the SPI1- lon - fliC mutant enabled the serological differentiation of vaccinated and infected chickens. The absence of fliC therefore did not affect the immunogenicity of the vaccine strain and allowed for serological differentiation of the vaccinated chickens. The SPI1- lon - fliC mutant is therefore a suitable marker vaccine strain for oral vaccination of poultry.