Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco | Zendy

Guanzheng Qu | Zendy; Tangchun Zheng | Zendy; Guifeng Liu | Zendy; Wenjie Wang | Zendy; Lina Zang | Zendy; Huanzhen Liu | Zendy; Chuanping Yang | Zendy

Open Access

Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco

Author(s) -

Guanzheng Qu,

Tangchun Zheng,

Guifeng Liu,

Wenjie Wang,

Lina Zang,

Huanzhen Liu,

Chuanping Yang

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0063398

Subject(s) - betula platyphylla , mads box , arabidopsis , biology , transgene , sepal , ectopic expression , chloroplast , botany , genetically modified crops , methyl jasmonate , gene , genetics , stamen , pollen , mutant

In this study, a MADS-box gene ( BpMADS ), which is an ortholog of AP1 from Arabidopsis , was isolated from birch ( Betula platyphylla ). Transgenic Arabidopsis containing a BpMADS promoter::GUS construct was produced, which exhibited strong GUS staining in sepal tissues. Ectopic expression of BpMADS significantly enhanced the flowering of tobacco (35S::BpMADS). In addition, the chloroplasts of transgenic tobacco exhibited much higher growth and division rates, as well rates of photosynthesis, than wild-type. A grafting experiment demonstrated that the flowering time of the scion was not affected by stock that overexpressed BpMADS . In addition, the overexpression of BpMADS resulted in the upregulation of some flowering-related genes in tobacco.

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