Open AccessThe Role of hnRPUL1 Involved in DNA Damage Response Is Related to PARP1
Author(s) -
Hong Zhang,
Jie Jiang,
Junfeng Ma,
Shikui Dai,
Ting Xu,
Hui Li,
Akira Yasui
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0060208
Subject(s) - parp1 , dna damage , gene knockdown , biology , dna , dna repair , microbiology and biotechnology , genetics , gene , poly adp ribose polymerase , polymerase
Heterogeneous nuclear ribonucleoprotein U-like 1 (hnRPUL1) -also known as adenovirus early region 1B-associated proteins 5 (E1B-AP5) - plays a role in RNA metabolism. Recently, hnRPUL1 has also been shown to be involved in DNA damage response, but the function of hnRPUL1 in response to DNA damage remains unclear. Here, we have demonstrated that hnRPUL1 is associated with PARP1 and recruited to DNA double-strand breaks (DSBs) sites in a PARP1-mediated poly (ADP-ribosyl) ation dependent manner. In turn, hnRPUL1 knockdown enhances the recruitment of PARP1 to DSBs sites. Specifically, we showed that hnRPUL1 is also implicated in the transcriptional regulation of PARP1 gene. Thus, we propose hnRPUL1 as a new component related to PARP1 in DNA damage response and repair.