Open AccessPsVPS1, a Dynamin-Related Protein, Is Involved in Cyst Germination and Soybean Infection of Phytophthora sojae
Author(s) -
Delong Li,
Zhijian Zhao,
Yidan Huang,
Zhaojun Lu,
Meng Ye,
Yujuan Hao,
Chunhua Zhai,
Yuanchao Wang
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0058623
Subject(s) - phytophthora sojae , vacuole , biology , microbiology and biotechnology , effector , secretion , golgi apparatus , mutant , gene , biochemistry , pathogen , endoplasmic reticulum , cytoplasm
Plant pathogens secrete effector proteins to suppress plant immunity. However, the mechanism by which oomycete pathogens deliver effector proteins during plant infection remains unknown. In this report, we characterized a Phytophthora sojae vps1 gene. This gene encodes a homolog of the Saccharomyces cerevisiae vacuolar protein sorting gene vps1 that mediates budding of clathrin-coated vesicles from the late Golgi, which are diverted from the general secretory pathway to the vacuole. PsVPS1 -silenced mutants were generated using polyethylene glycol-mediated protoplast stable transformation and were viable but had reduced extracellular protein activity. The PsVPS1 -silenced mutants showed impaired hyphal growth, and the shapes of the vacuoles were highly fragmented. Silencing of PsVPS1 affected cyst germination as well as the polarized growth of germinated cysts. Silenced mutants showed impaired invasion of susceptible soybean plants regardless of wounding. These results suggest that PsVPS1 is involved in vacuole morphology and cyst development. Moreover, it is essential for the virulence of P. sojae and extracellular protein secretion.