Open AccessRapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells
Author(s) -
Eva Potthoff,
Orane GuillaumeGentil,
Dario Ossola,
Jérôme Polesel-Maris,
Salomé LeibundGutLandmann,
Tomaso Zambelli,
Julia A. Vorholt
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0052712
Subject(s) - force spectroscopy , adhesion , cell adhesion , biophysics , fibronectin , chemistry , microbiology and biotechnology , yeast , atomic force microscopy , cell , biology , nanotechnology , materials science , biochemistry , organic chemistry
Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of adhesion forces of different cell types using a single probe. The pace of single-cell force-spectroscopy was accelerated to up to 200 yeast and 20 mammalian cells per probe when replacing the conventional cell trapping cantilever chemistry of atomic force microscopy by underpressure immobilization with fluidic force microscopy (FluidFM). In consequence, statistically relevant data could be recorded in a rapid manner, the spectrum of examinable cells was enlarged, and the cell physiology preserved until approached for force spectroscopy. Adhesion forces of Candida albicans increased from below 4 up to 16 nN at 37°C on hydrophobic surfaces, whereas a Δ hgc1 -mutant showed forces consistently below 4 nN. Monitoring adhesion of mammalian cells revealed mean adhesion forces of 600 nN of HeLa cells on fibronectin and were one order of magnitude higher than those observed for HEK cells.