Open AccessEnantioselective Induction of a Glutathione-S-Transferase, a Glutathione Transporter and an ABC Transporter in Maize by Metolachlor and Its (S)-Isomer
Author(s) -
Sen Pang,
Zhaojin Ran,
Zhiqian Liu,
Xiaoyu Song,
Duan Liu,
Xuefeng Liu,
Chengju Wang
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0048085
Subject(s) - metolachlor , glutathione , transporter , glutathione s transferase , atp binding cassette transporter , chemistry , biochemistry , glutathione transferase , metabolism , biology , enzyme , gene , pesticide , agronomy , atrazine
The metabolism of chiral herbicides in plants remains poorly understood. Glutathione conjugation reactions are one of the principal mechanisms that plants utilize to detoxify xenobiotics. The induction by rac - and S -metolachlor of the expression of three genes, ZmGST27 , ZmGT1 and ZmMRP1 , encoding respectively a glutathione- S -transferase, a glutathione transporter and an ATP-binding cassette (ABC) transporter was studied in maize. The results demonstrate that the inducing effect of rac - and S -metolachlor on the expression of ZmGST27 and ZmGT1 is comparable. However, the inducing effect of rac -metolachlor on ZmMRP1 expression is more pronounced than that of S -metolachlor. Furthermore, vanadate, an ABC transporter inhibitor, could greatly reduce the difference in herbicidal activity between rac - and S -metolachlor. These results suggest that the ABC transporters may preferentially transport conjugates of rac -metolachlor, leading to a faster metabolism of the latter. Through comparing the expression of ZmGST27 , ZmMRP1 and ZmGT1 after treatment by rac - and S -metolachlor, we provide novel insights into the metabolic processes of chiral herbicides in plants.