Open AccessIgG Expression in Human Colorectal Cancer and Its Relationship to Cancer Cell Behaviors
Author(s) -
iu,
Jie Zhang,
Tao Huang,
Yingui Sun,
Zhengshan Chen,
Weining Yi,
Christine Korteweg,
Juping Wang,
Jiang Gu
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0047362
Subject(s) - colorectal cancer , cancer , cancer research , immune system , cancer cell , cyclin d1 , lymph node , biology , mouse model of colorectal and intestinal cancer , immunology , medicine , cell cycle
Increasing evidence indicates that various cancer cell types are capable of producing IgG. The exact function of cancer-derived IgG has, however, not been elucidated. Here we demonstrated the expression of IgG genes with V(D)J recombination in 80 cases of colorectal cancers, 4 colon cancer cell lines and a tumor bearing immune deficient mouse model. IgG expression was associated with tumor differentiation, pTNM stage, lymph node involvement and inflammatory infiltration and positively correlated with the expressions of Cyclin D1, NF-κB and PCNA. Furthermore, we investigated the effect of cancer-derived IgG on the malignant behaviors of colorectal cancer cells and showed that blockage of IgG resulted in increased apoptosis and negatively affected the potential for anchor-independent colony formation and cancer cell invasion. These findings suggest that IgG synthesized by colorectal cancer cells is involved in the development and growth of colorectal cancer and blockage of IgG may be a potential therapy in treating this cancer.