Open AccessPhosphorylation-Dependent SUMOylation of the Transcription Factor NF-E2
Author(s) -
Yee Fun Su,
Yu-Chiau Shyu,
Che Kun James Shen,
Jiann Loung Hwang
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0044608
Subject(s) - sumo protein , phosphorylation , transcription factor , microbiology and biotechnology , activator (genetics) , protein kinase a , chemistry , transcription (linguistics) , biology , biochemistry , ubiquitin , gene , linguistics , philosophy
Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.