Open AccessSystematic Functional Characterization of Cytochrome P450 2E1 Promoter Variants in the Chinese Han Population
Author(s) -
Xunyi Huang,
Lili Chen,
Wenlong Song,
Ling Chen,
Jiamin Niu,
Xuelei Han,
Feng Gao,
Lin He,
Shengying Qin
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0040883
Subject(s) - single nucleotide polymorphism , haplotype , luciferase , genetics , biology , snp , promoter , gene , microbiology and biotechnology , allele , genotype , gene expression , transfection
CYP2E1 promoter polymorphisms can lead to significant interindividual differences in expression of CYP2E1. Using a database of CYP2E1 gene polymorphisms established in 2010, our study aimed to functionally characterize the single nucleotide polymorphisms (SNPs) of the promoter region and corresponding haplotypes in the Chinese Han population. Six novel SNPs and seven haplotypes with a frequency equal to or greater than 0.01 were constructed on a luciferase reporter system on the basis of site-directed mutagenesis. Dual luciferase reporter systems were used to analyze regulatory activity. The constructs including single novel SNP mutations exhibited insignificant change in luciferase activity, whereas, the activity produced by Haplo1( GTTGCTATAT ), Haplo2 ( CTTGCTATAT ) and Haplo7 ( GAGCTCACAT ), containing a −333T>A polymorphism was significantly greater than for the wild type in Hep G2 cells (p<0.05), being 1.5−, 2.0− and 1.4− times greater respectively. These findings suggest the possibility of significant clinical prediction of adverse drug reaction and the facilitation of personalized medicine.