Open AccessPtdIns (3,4,5) P3 Recruitment of Myo10 Is Essential for Axon Development
Author(s) -
Huali Yu,
Nannan Wang,
XingDa Ju,
Yan Mei Yang,
Dong Sun,
Mingming Lai,
Lei Cui,
Muhammad Abid Sheikh,
Jianhua Zhang,
Xingzhi Wang,
Xinen Zhu
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0036988
Subject(s) - microbiology and biotechnology , biology , axon , axon guidance , pleckstrin homology domain , rhoa , ectopic expression , neurite , growth cone , signal transduction , neuroscience , cell culture , genetics , in vitro
Myosin X (Myo10) with pleckstrin homology (PH) domains is a motor protein acting in filopodium initiation and extension. However, its potential role has not been fully understood, especially in neuronal development. In the present study the preferential accumulation of Myo10 in axon tips has been revealed in primary culture of hippocampal neurons with the aid of immunofluorescence from anti-Myo10 antibody in combination with anti-Tuj1 antibody as specific marker. Knocking down Myo10 gene transcription impaired outgrowth of axon with loss of Tau-1-positive phenotype. Interestingly, inhibition of actin polymerization by cytochalasin D rescued the defect of axon outgrowth. Furthermore, ectopic expression of Myo10 with enhanced green fluorescence protein (EGFP) labeled Myo10 mutants induced multiple axon-like neurites in a motor-independent way. Mechanism studies demonstrated that the recruitment of Myo10 through its PH domain to phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5) P3) was essential for axon formation. In addition, in vivo studies confirmed that Myo10 was required for neuronal morphological transition during radial neuronal migration in the developmental neocortex.