Open AccessDifferent Capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles
Author(s) -
Marcus Settles,
Martin Etzrodt,
Katja Kosanke,
Matthias Schiemann,
Alexander Zimmermann,
Reinhard Meier,
Rickmer Braren,
Armin Huber,
Ernst J. Rummeny,
Ralph Weissleder,
Filip K. Świrski,
Moritz Wildgruber
Publication year - 2011
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0025197
Subject(s) - cd16 , cd14 , iron oxide nanoparticles , monocyte , flow cytometry , nanotoxicology , biophysics , nanoparticle , phagocytosis , chemistry , microbiology and biotechnology , materials science , biology , nanotechnology , immunology , immune system , cd3 , cd8
Objective To explore the capacity of human CD14 + CD16 ++ and CD14 ++ CD16 - monocytes to phagocyte iron-oxide nanoparticles in vitro. Methods Human monocytes were labeled with four different magnetic nanoparticle preparations (Ferumoxides, SHU 555C, CLIO-680, MION-48) exhibiting distinct properties and cellular uptake was quantitatively assessed by flow cytometry, fluorescence microscopy, atomic absorption spectrometry and Magnetic Resonance Imaging (MRI). Additionally we determined whether cellular uptake of the nanoparticles resulted in phenotypic changes of cell surface markers. Results Cellular uptake differed between the four nanoparticle preparations. However for each nanoparticle tested, CD14 ++ CD16 - monocytes displayed a significantly higher uptake compared to CD14 + CD16 ++ monocytes, this resulted in significantly lower T1 and T2 relaxation times of these cells. The uptake of iron-oxide nanoparticles further resulted in a remarkable shift of expression of cell surface proteins indicating that the labeling procedure affects the phenotype of CD14 + CD16 ++ and CD14 ++ CD16 - monocytes differently. Conclusion Human monocyte subsets internalize different magnetic nanoparticle preparations differently, resulting in variable loading capacities, imaging phenotypes and likely biological properties.