Open AccessTranscriptome Analysis of MSC and MSC-Derived Osteoblasts on Resomer® LT706 and PCL: Impact of Biomaterial Substrate on Osteogenic Differentiation
Author(s) -
Sabine Neuß,
Bernd Denecke,
Lin Gan,
Qiong Lin,
Manfred Bovi,
Christian Apel,
Michael Wöltje,
Anandhan Dhanasingh,
Jochen Salber,
Ruth Knüchel,
Martin Zenke
Publication year - 2011
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0023195
Subject(s) - mesenchymal stem cell , tissue engineering , microbiology and biotechnology , scaffold , biomedical engineering , biomaterial , cellular differentiation , chemistry , bone tissue , biology , biochemistry , medicine , gene
Background Mesenchymal stem cells (MSC) represent a particularly attractive cell type for bone tissue engineering because of their ex vivo expansion potential and multipotent differentiation capacity. MSC are readily differentiated towards mature osteoblasts with well-established protocols. However, tissue engineering frequently involves three-dimensional scaffolds which (i) allow for cell adhesion in a spatial environment and (ii) meet application-specific criteria, such as stiffness, degradability and biocompatibility. Methodology/Principal Findings In the present study, we analysed two synthetic, long-term degradable polymers for their impact on MSC-based bone tissue engineering: PLLA-co-TMC (Resomer® LT706) and poly(ε-caprolactone) (PCL). Both polymers enhance the osteogenic differentiation compared to tissue culture polystyrene (TCPS) as determined by Alizarin red stainings, scanning electron microscopy, PCR and whole genome expression analysis. Resomer® LT706 and PCL differ in their influence on gene expression, with Resomer® LT706 being more potent in supporting osteogenic differentiation of MSC. The major trigger on the osteogenic fate, however, is from osteogenic induction medium. Conclusion This study demonstrates an enhanced osteogenic differentiation of MSC on Resomer® LT706 and PCL compared to TCPS. MSC cultured on Resomer® LT706 showed higher numbers of genes involved in skeletal development and bone formation. This identifies Resomer® LT706 as particularly attractive scaffold material for bone tissue engineering.