Open AccessEnzyme-Linked Immunosorbent Assay-Format Tissue Culture Infectious Dose-50 Test for Titrating Dengue Virus
Author(s) -
Jie Li,
Dongmei Hu,
Xiaoyi Ding,
Yue Chen,
Yuxian Pan,
Li-wen Qiu,
Xin Che
Publication year - 2011
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0022553
Subject(s) - dengue virus , vero cell , titer , cytopathic effect , virology , dengue fever , titration , virus quantification , biology , virus , microbiology and biotechnology , chemistry , inorganic chemistry
A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID 50 ) test (TCID 50 -ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID 50 -ELISA were comparable to those obtained by the plaque assay and by the traditional TCID 50 -cytopathic effect (CPE) test (TCID 50 -CPE), with a better reproducibility and a lower coefficient of variation. Quantitative comparison of TCID 50 -ELISA and TCID 50 -CPE resulted in a correlation coefficient of 0.976. Moreover, this new method showed a wider application to C6/36, Vero E6, BHK-21, and Vero cells compared with other titration methods. In summary, the novel TCID 50 -ELISA method described here provides a more reliable and more accurate alternative compared to the plaque assay and TCID 50 -CPE for titration of dengue virus.