Open AccessForce Measurements of TCR/pMHC Recognition at T Cell Surface
Author(s) -
Pierre-Henri Puech,
Damien Névoltris,
P. Robert,
Laurent Limozin,
Catherine Boyer,
Pierre Bongrand
Publication year - 2011
Publication title -
hal (le centre pour la communication scientifique directe)
Language(s) - English
DOI - 10.1371/journal.pone.0022344.s005
Subject(s) - t cell receptor , surface (topology) , t cell , computer science , computational biology , biology , immunology , mathematics , immune system , geometry
International audienceThe rupture forces and adhesion frequencies of single recognition complexes between an affinityselected peptide/MHC complex and a TCR at a murine hybridoma surface were measured usingAtomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends onthe nature of the peptide but the rupture force does not. When CD8 is present, no effect of the natureof the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorterTCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, suchexperiments could help the dissection of the sequential steps by which the TCR reads thepeptide/MHC complex in order to control T cell activation