Open AccessAedes aegypti abundance and insecticide resistance profiles in the Applying Wolbachia to Eliminate Dengue trial
Author(s) -
Warsito Tantowijoyo,
Stephanie K. Tanamas,
Indah Nurhayati,
Sigit Setyawan,
Nida Budiwati,
Iva Fitriana,
Inggrid Ernesia,
Dwi Satria Wardana,
Endah Supriyati,
Eggi Arguni,
Yeti Meitika,
Equatori Prabowo,
Bekti Andari,
Benjamin R. Green,
Lauren Hodgson,
Edwige Rancès,
Peter Ryan,
Scott L. O’Neill,
Katherine L. Anders,
Muhammad Ridwan Ansari,
Citra Indriani,
Riris Andono Ahmad,
Adi Utarini,
Cameron P. Simmons
Publication year - 2022
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0010284
Subject(s) - wolbachia , aedes aegypti , dengue fever , insecticide resistance , dengue virus , aedes , biology , virology , chikungunya , toxicology , veterinary medicine , ecology , medicine , larva , host (biology)
The Applying Wolbachia to Eliminate Dengue (AWED) trial was a parallel cluster randomised trial that demonstrated Wolbachia ( w Mel) introgression into Ae . aegypti populations reduced dengue incidence. In this predefined substudy, we compared between treatment arms, the relative abundance of Ae . aegypti and Ae . albopictus before, during and after w Mel-introgression. Between March 2015 and March 2020, 60,084 BG trap collections yielded 478,254 Ae . aegypti and 17,623 Ae . albopictus . Between treatment arms there was no measurable difference in Ae . aegypti relative abundance before or after w Mel-deployments, with a count ratio of 0.96 (95% CI 0.76, 1.21) and 1.00 (95% CI 0.85, 1.17) respectively. More Ae . aegypti were caught per trap per week in the w Mel-intervention arm compared to the control arm during w Mel deployments (count ratio 1.23 (95% CI 1.03, 1.46)). Between treatment arms there was no measurable difference in the Ae . albopictus population size before, during or after w Mel-deployment (overall count ratio 1.10 (95% CI 0.89, 1.35)). We also compared insecticide resistance phenotypes of Ae . aegypti in the first and second years after w Mel-deployments. Ae . aegypti field populations from w Mel-treated and untreated arms were similarly resistant to malathion (0.8%), permethrin (1.25%) and cyfluthrin (0.15%) in year 1 and year 2 of the trial. In summary, we found no between-arm differences in the relative abundance of Ae . aegypti or Ae . albopictus prior to or after w Mel introgression, and no between-arm difference in Ae . aegypti insecticide resistance phenotypes. These data suggest neither Aedes abundance, nor insecticide resistance, confounded the epidemiological outcomes of the AWED trial.