Open AccessRecombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera
Author(s) -
Yan Liu,
Ning Xu,
Yansong Li,
Bin Tang,
Hualin Yang,
Weihua Gao,
Mingyuan Liu,
Xiaolei Liu,
Yu Zhou
Publication year - 2021
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0009723
Subject(s) - trichinella spiralis , trichinosis , virology , antibody , biology , recombinant dna , parasite hosting , trichinella , immunology , antigen , helminths , biochemistry , world wide web , gene , computer science
Objectives Trichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T . spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent needed for monitoring T . spiralis exposure. Methods A competition enzyme-linked immunosorbent assay (cELISA) for T . spiralis infection diagnosis in multihost sera was developed based on recombinant cystatin-like protein (rCLP-cELISA) as well as monoclonal antibodies. The sensitivity and accuracy of the rCLP-cELISA were quantified using swine (n = 1316), mice (n = 189) and human (n = 157) serum samples. T . spiralis -antibody targeting test ability of the rCLP-cELISA in swine (n = 22) and human (n = 36), instead of other parasites or viruses antibodies, was evaluated. Results The rCLP-cELISA showed high agreement with commercial ELISA kits in field swine sera assessed by Cohen’s kappa value (κ = 0.7963). And it showed 100% specificity in human trichinellosis detection with sensitivity of 96.49%, no cross-reaction with other parasite or virus infections, and high positive detection rate of 87.5% in low-dose infected swine. Besides, the rCLP-cELISA exhibited potential in the detection of T . spiralis , T . nelsoni and Trichinella T8 infections. Conclusions The rCLP-cELISA can be used for T . spiralis -associated antibody test in multihost sera.