Open AccessInsecticide resistance in dengue vectors from hotspots in Selangor, Malaysia
Author(s) -
Rosilawati Rasli,
Young-Keun Cheong,
Mohd Khairuddin Che Ibrahim,
Siti Futri Farahininajua Fikri,
Rusydi Najmuddin Norzali,
Nur Ayuni Nazarudin,
Nur Fadillah Hamdan,
Khairul Asuad Muhamed,
Afiq Ahnaf Hafisool,
Ruziyatul Aznieda Azmi,
Harith Aswad Ismail,
Roziah Ali,
Nurulhusna Ab Hamid,
Mohd Zainuldin Taib,
Topek Omar,
Nazni Wasi Ahmad,
Hanlim Lee
Publication year - 2021
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0009205
Subject(s) - dengue fever , insecticide resistance , dengue virus , environmental health , biology , geography , virology , toxicology , medicine
Background In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.