Open AccessSerodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Author(s) -
Pyo Yun Cho,
Ji Yun Lee,
Tae Im Kim,
Jin Ho Song,
Sung Jong Hong,
Won Gi Yoo,
Takafumi Tsuboi,
Kwon Soo Ha,
Jae Wan Jung,
Satoru Takeo,
Eun Taek Han,
Banchob Sripa,
Sung Tae Hong,
Jong Yil Chai,
Ho Woo Nam,
Jhang Ho Pak,
Tong Soo Kim
Publication year - 2020
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0008998
Subject(s) - clonorchis sinensis , clonorchiasis , opisthorchis viverrini , antigen , antigenicity , biology , virology , opisthorchiasis , recombinant dna , fusion protein , cross reactivity , liver fluke , microbiology and biotechnology , immunology , helminths , gene , medicine , cross reactions , pathology , biochemistry
Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C . sinensis -infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini -infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.