Open AccessTransmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses
Author(s) -
Guoguo Ye,
Yujuan Wang,
Xiaoyun Liu,
Qiannan Dong,
Quanxing Cai,
Zhiming Yuan,
Han Xia
Publication year - 2020
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0008920
Subject(s) - biology , aedes albopictus , infectivity , virology , virus , zika virus , culex quinquefasciatus , transmission (telecommunications) , midgut , dengue virus , arbovirus , larva , aedes aegypti , ecology , electrical engineering , engineering
Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on arboviruses. Mesonivirus is a class of ISVs with a wide geographical distribution. Recently, our laboratory reported the isolation of a novel strain of mesonivirus, Yichang virus (YCV), from Culex mosquitoes, China. In this study, the experimental infection of YCV by the oral route for adult and larvae mosquitoes, and the vertical transmission has been conducted, which suggests that YCV could adopt a mixed-mode transmission. Controlled experiments showed that the infectivity of YCV depends on the mosquito species, virus dose, and infection route. The proliferation curve and tissue distribution of YCV in Cx . quinquefasciatus and Ae . albopictus showed that YCV is more susceptible to Ae . albopictus and is located in the midgut. Furthermore, we also assessed the interference of YCV with flaviviruses both in vitro and in vivo . YCV significantly inhibited the proliferation of DENV-2 and ZIKV, in cell culture, and reduced transmission rate of DENV-2 in Ae . albopictus . Our work provides insights into the transmission of ISVs in different mosquito species during ontogeny and their potential ability to interact with mosquito-borne viruses.