Open AccessEvaluation of fecal immunoassays for canine Echinococcus infection in China
Author(s) -
Liying Wang,
Qian Wang,
Huixia Cai,
Hu Wang,
Yan Huang,
Yue Feng,
Xia Bai,
Min Qin,
Sylvie Manguin,
Laurent Gavotte,
Wei Wu,
Roger Frutos
Publication year - 2021
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0008690
Subject(s) - echinococcosis , echinococcus , feces , gold standard (test) , veterinary medicine , immunoassay , medicine , biology , virology , immunology , pathology , antibody , microbiology and biotechnology
Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis.