Open AccessLiver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment
Author(s) -
Vitor Hugo Simões Miranda,
Talita Rocha Gomes,
Dirli Emerick Eller,
Lorena de Cássia Neres Ferraz,
Ana Cristina Chaves,
Kelly Alves Bicalho,
Carlos Eduardo Calzavara-Silva,
Alexander Birbrair,
Marcelo Antônio Pascoal Xavier,
Alfredo M. Góes,
Rodrigo Corrêa-Oliveira,
Érica Alessandra Rocha Alves,
Adriana Bozzi
Publication year - 2020
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0008635
Subject(s) - praziquantel , schistosomiasis , schistosoma mansoni , mesenchymal stem cell , adipose tissue , stem cell , immunology , granuloma , inflammation , medicine , pathology , biology , stem cell therapy , transplantation , helminths , genetics
Background In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment. Methodology/Prinicpal findings C57BL/6 mice infected with S . mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4 + T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice. Conclusions Our results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S . mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis.