Open AccessEvidence of the absence of human African trypanosomiasis in two northern districts of Uganda: Analyses of cattle, pigs and tsetse flies for the presence of Trypanosoma brucei gambiense
Author(s) -
Lucas J. Cunningham,
Jessica Lingley,
Iñaki Tirados,
Johan Esterhuizen,
Mercy Opiyo,
Clement T. N. Mangwiro,
Mike Lehane,
Stephen J. Torr
Publication year - 2020
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0007737
Subject(s) - biology , trypanosomiasis , african trypanosomiasis , trypanosoma brucei , trypanosoma , veterinary medicine , virology , transmission (telecommunications) , parasitology , cattle diseases , zoology , medicine , biochemistry , electrical engineering , gene , engineering
Background Large-scale control of sleeping sickness has led to a decline in the number of cases of Gambian human African trypanosomiasis (g-HAT) to <2000/year. However, achieving complete and lasting interruption of transmission may be difficult because animals may act as reservoir hosts for T . b . gambiense . Our study aims to update our understanding of T . b . gambiense in local vectors and domestic animals of N.W. Uganda. Methods We collected blood from 2896 cattle and 400 pigs and In addition, 6664 tsetse underwent microscopical examination for the presence of trypanosomes. Trypanosoma species were identified in tsetse from a subsample of 2184 using PCR. Primers specific for T . brucei s.l. and for T . brucei sub-species were used to screen cattle, pig and tsetse samples. Results In total, 39/2,088 (1.9%; 95% CI = 1.9–2.5) cattle, 25/400 (6.3%; 95% CI = 4.1–9.1) pigs and 40/2,184 (1.8%; 95% CI = 1.3–2.5) tsetse, were positive for T . brucei s.l.. Of these samples 24 cattle (61.5%), 15 pig (60%) and 25 tsetse (62.5%) samples had sufficient DNA to be screened using the T . brucei sub-species PCR. Further analysis found no cattle or pigs positive for T . b . gambiense , however, 17/40 of the tsetse samples produced a band suggestive of T . b . gambiense . When three of these 17 PCR products were sequenced the sequences were markedly different to T . b . gambiense , indicating that these flies were not infected with T . b . gambiense . Conclusion The lack of T . b . gambiense positives in cattle, pigs and tsetse accords with the low prevalence of g-HAT in the human population. We found no evidence that livestock are acting as reservoir hosts. However, this study highlights the limitations of current methods of detecting and identifying T . b . gambiense which relies on a single copy-gene to discriminate between the different sub-species of T . brucei s.l.