Open AccessThe detection of cryptic Plasmodium infection among villagers in Attapeu province, Lao PDR
Author(s) -
Moritoshi Iwagami,
Sengdeuane Keomalaphet,
Phonepadith Khattignavong,
Pheovaly Soundala,
Lavy Lorphachan,
Emilie Louise Akiko Matsumoto-Takahashi,
M Strobel,
Daniel Reinharz,
Manisack Phommasansack,
Bouasy Hongvanthong,
Paul T. Brey,
Shigeyuki Kano
Publication year - 2017
Publication title -
plos neglected tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.99
H-Index - 135
eISSN - 1935-2735
pISSN - 1935-2727
DOI - 10.1371/journal.pntd.0006148
Subject(s) - malaria , parasitemia , plasmodium vivax , plasmodium falciparum , asymptomatic , plasmodium (life cycle) , parasitology , tropical medicine , medicine , biology , virology , immunology , parasite hosting , pathology , world wide web , computer science
Background Although the malaria burden in the Lao PDR has gradually decreased, the elimination of malaria by 2030 presents many challenges. Microscopy and malaria rapid diagnostic tests (RDTs) are used to diagnose malaria in the Lao PDR; however, some studies have reported the prevalence of sub-microscopic Plasmodium infections or asymptomatic Plasmodium carriers in endemic areas. Thus, highly sensitive detection methods are needed to understand the precise malaria situation in these areas. Methodology/Principal findings A cross-sectional malaria field survey was conducted in 3 highly endemic malaria districts (Xaysetha, Sanamxay, Phouvong) in Attapeu province, Lao PDR in 2015, to investigate the precise malaria endemicity in the area; 719 volunteers from these villages participated in the survey. Microscopy, RDTs and a real-time nested PCR were used to detect Plasmodium infections and their results were compared. A questionnaire survey of all participants was also conducted to estimate risk factors of Plasmodium infection. Numbers of infections detected by the three methods were microscopy: P . falciparum (n = 1), P . vivax (n = 2); RDTs: P . falciparum (n = 2), P . vivax (n = 3); PCR: Plasmodium (n = 47; P . falciparum [n = 4], P . vivax [n = 41], mixed infection [n = 2]; 6.5%, 47/719). Using PCR as a reference, the sensitivity and specificity of microscopy were 33.3% and 100.0%, respectively, for detecting P . falciparum infection, and 7.0% and 100.0%, for detecting P . vivax infection. Among the 47 participants with parasitemia, only one had a fever (≥37.5°C) and 31 (66.0%) were adult males. Risk factors of Plasmodium infection were males and soldiers, whereas a risk factor of asymptomatic Plasmodium infection was a history of ≥3 malaria episodes. Conclusions/Significance There were many asymptomatic Plasmodium carriers in the study areas of Attapeu province in 2015. Adult males, probably soldiers, were at high risk for malaria infection. P . vivax , the dominant species, accounted for 87.2% of the Plasmodium infections among the participants. To achieve malaria elimination in the Lao PDR, highly sensitive diagnostic tests, including PCR-based diagnostic methods should be used, and plans targeting high-risk populations and elimination of P . vivax should be designed and implemented.