Open AccessThe genome variation and developmental transcriptome maps reveal genetic differentiation of skeletal muscle in pigs
Author(s) -
Yalan Yang,
Junyu Yan,
Xinhao Fan,
Jiaxing Chen,
Zishuai Wang,
Xiaoqin Liu,
Guoqiang Yi,
Yuwen Liu,
Yongchao Niu,
Longchao Zhang,
Lixian Wang,
Shuai Cheng Li,
Kui Li,
Zhonglin Tang
Publication year - 2021
Publication title -
plos genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.587
H-Index - 233
eISSN - 1553-7404
pISSN - 1553-7390
DOI - 10.1371/journal.pgen.1009910
Subject(s) - biology , transcriptome , skeletal muscle , myocyte , phenotype , genetics , obscurin , gene expression profiling , microbiology and biotechnology , gene , gene expression , endocrinology , titin , sarcomere
Natural and artificial directional selections have resulted in significantly genetic and phenotypic differences across breeds in domestic animals. However, the molecular regulation of skeletal muscle diversity remains largely unknown. Here, we conducted transcriptome profiling of skeletal muscle across 27 time points, and performed whole-genome re-sequencing in Landrace (lean-type) and Tongcheng (obese-type) pigs. The transcription activity decreased with development, and the high-resolution transcriptome precisely captured the characterizations of skeletal muscle with distinct biological events in four developmental phases: Embryonic, Fetal, Neonatal, and Adult. A divergence in the developmental timing and asynchronous development between the two breeds was observed; Landrace showed a developmental lag and stronger abilities of myoblast proliferation and cell migration, whereas Tongcheng had higher ATP synthase activity in postnatal periods. The miR-24-3p driven network targeting insulin signaling pathway regulated glucose metabolism. Notably, integrated analysis suggested SATB2 and XLOC_036765 contributed to skeletal muscle diversity via regulating the myoblast migration and proliferation, respectively. Overall, our results provide insights into the molecular regulation of skeletal muscle development and diversity in mammals.