Open AccessRegulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA
Author(s) -
Bin Zhu,
Linhong Li,
Rui Wei,
Pei Liang,
Xiwu Gao
Publication year - 2021
Publication title -
plos genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.587
H-Index - 233
eISSN - 1553-7404
pISSN - 1553-7390
DOI - 10.1371/journal.pgen.1009888
Subject(s) - biology , plutella , microrna , insecticide resistance , gene , rna , genetics , untranslated region , gene expression , regulation of gene expression , toxicology , botany , lepidoptera genitalia
The evolution of resistance to insecticides is well known to be closely associated with the overexpression of detoxifying enzymes. Although the role of glutathione S -transferase (GST) genes in insecticide resistance has been widely reported, the underlying regulatory mechanisms are poorly understood. Here, one GST gene ( GSTu1 ) and its antisense transcript (lnc- GSTu1 -AS) were identified and cloned, and both of them were upregulated in several chlorantraniliprole-resistant Plutella xylostella populations. GSTu1 was confirmed to be involved in chlorantraniliprole resistance by direct degradation of this insecticide. Furthermore, we demonstrated that lnc- GSTu1 -AS interacted with GSTu1 by forming an RNA duplex, which masked the binding site of miR-8525-5p at the GSTu1 -3′UTR. In summary, we revealed that lnc- GSTu1 -AS maintained the mRNA stability of GSTu1 by preventing its degradation that could have been induced by miR-8525-5p and thus increased the resistance of P . xylostella to chlorantraniliprole. Our findings reveal a new noncoding RNA-mediated pathway that regulates the expression of detoxifying enzymes in insecticide-resistant insects and offer opportunities for the further understanding of the mechanisms of insecticide and drug resistance.