Open AccessChromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans
Author(s) -
Eric J. Foss,
Smitha Sripathy,
Tonibelle Gatbonton-Schwager,
Hyunchang Kwak,
Adam H. Thiesen,
Uyen Lao,
Antonio Bedalov
Publication year - 2021
Publication title -
plos genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.587
H-Index - 233
eISSN - 1553-7404
pISSN - 1553-7390
DOI - 10.1371/journal.pgen.1009714
Subject(s) - biology , origin of replication , genetics , genome , control of chromosome duplication , origin recognition complex , dna replication , pre replication complex , chromosome , replication (statistics) , replication timing , dna , gene , eukaryotic dna replication , virology
The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed similar individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution: Footprints in budding yeast were more sharply focused compared to the other three organisms, consistent with the relative sequence specificity of replication origins in S . cerevisiae . Nonetheless, with some clear exceptions, most notably the inactive X-chromosome, much of the fluctuation in replication timing along the chromosomes in all four organisms reflected uneven chromosomal distribution of pre-replication complexes.