z-logo
open-access-imgOpen Access
DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans
Author(s) -
Hui Hong,
Huicheng Chen,
Yuxia Zhang,
Zhimao Wu,
Yingying Zhang,
Yingyi Zhang,
Hu Zeng,
Jian V. Zhang,
Kun Ling,
Jinghua Hu,
Qing Wei
Publication year - 2021
Publication title -
plos genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.587
H-Index - 233
eISSN - 1553-7404
pISSN - 1553-7390
DOI - 10.1371/journal.pgen.1009618
Subject(s) - ciliogenesis , biology , microbiology and biotechnology , mutant , patched , regulator , caenorhabditis elegans , neurite , mutagenesis , genetics , cilium , signal transduction , hedgehog signaling pathway , gene , in vitro
Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C . elegans , we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here