Open AccessRNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes
Author(s) -
Alexis H Bennett,
Marie-Françoise O’Donohue,
Stacey R. Gundry,
A CHAN,
Jeffrey J. Widrick,
Isabelle Draper,
Anirban Chakraborty,
Yi Zhou,
Leonard I. Zon,
PierreEmmanuel Gleizes,
Alan H. Beggs,
Vandana Gupta
Publication year - 2018
Publication title -
plos genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.587
H-Index - 233
eISSN - 1553-7404
pISSN - 1553-7390
DOI - 10.1371/journal.pgen.1007226
Subject(s) - myogenesis , biology , ribosome biogenesis , microbiology and biotechnology , rna helicase a , translational regulation , gene expression , ribosome , regulation of gene expression , post transcriptional regulation , rna , genetics , translation (biology) , gene , messenger rna , myocyte , helicase
Gene expression in a tissue-specific context depends on the combined efforts of epigenetic, transcriptional and post-transcriptional processes that lead to the production of specific proteins that are important determinants of cellular identity. Ribosomes are a central component of the protein biosynthesis machinery in cells; however, their regulatory roles in the translational control of gene expression in skeletal muscle remain to be defined. In a genetic screen to identify critical regulators of myogenesis, we identified a DEAD-Box RNA helicase, DDX27, that is required for skeletal muscle growth and regeneration. We demonstrate that DDX27 regulates ribosomal RNA (rRNA) maturation, and thereby the ribosome biogenesis and the translation of specific transcripts during myogenesis. These findings provide insight into the translational regulation of gene expression in myogenesis and suggest novel functions for ribosomes in regulating gene expression in skeletal muscles.