Open AccessExtracting multi-way chromatin contacts from Hi-C data
Author(s) -
Lei Liu,
Bokai Zhang,
Changbong Hyeon
Publication year - 2021
Publication title -
plos computational biology/plos computational biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.628
H-Index - 182
eISSN - 1553-7358
pISSN - 1553-734X
DOI - 10.1371/journal.pcbi.1009669
Subject(s) - chromatin , ctcf , chromosome conformation capture , chromosome , gaussian , complement (music) , sprite (computer graphics) , range (aeronautics) , computational biology , computer science , gene , algorithm , enhancer , statistical physics , physics , biology , genetics , artificial intelligence , gene expression , materials science , quantum mechanics , complementation , composite material , phenotype
There is a growing realization that multi-way chromatin contacts formed in chromosome structures are fundamental units of gene regulation. However, due to the paucity and complexity of such contacts, it is challenging to detect and identify them using experiments. Based on an assumption that chromosome structures can be mapped onto a network of Gaussian polymer, here we derive analytic expressions for n -body contact probabilities ( n > 2) among chromatin loci based on pairwise genomic contact frequencies available in Hi-C, and show that multi-way contact probability maps can in principle be extracted from Hi-C. The three-body (triplet) contact probabilities, calculated from our theory, are in good correlation with those from measurements including Tri-C, MC-4C and SPRITE. Maps of multi-way chromatin contacts calculated from our analytic expressions can not only complement experimental measurements, but also can offer better understanding of the related issues, such as cell-line dependent assemblies of multiple genes and enhancers to chromatin hubs, competition between long-range and short-range multi-way contacts, and condensates of multiple CTCF anchors.